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Efficacy and safety of various amphotericin B concentrations on Candida albicans in cold storage conditions

Cornea Dec 16, 2019

Tran KD, Aldrich BT, D'Amato Tóthová J, et al. - Researchers investigated amphotericin B concentration effective against Candida albicans contamination and safe for corneal endothelial cells (CECs) in cold storage conditions. Inoculation with 105 colony-forming units (CFUs)/mL of C. albicans (American Type Culture Collection 10231), supplementation with amphotericin B (0–20 μg/mL), and storage in cold conditions (2°C–8°C) for 72 hours was performed on triplicate media cultures, which were then examined quantitatively for CFUs. They ascertained C. albicans concentration in each sample at start and following 6, 24, 48, and 72 hours of storage. Findings revealed that amphotericin B showed optimal efficacy against C. albicans in cold storage conditions at concentrations ≥ 1.25 μg/mL, and 2.5 μg/mL decreased Candida contamination by > 90% following 6 hours of cold storage without losing CEC health.
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