Dingenen B, et al. - Experts intended to investigate test-retest reliability of single-leg hop tests in the forward, medial and rotational direction in non-injured athletes and ascertained the discriminative ability to discover leg asymmetries with these hop tests in an anterior cruciate ligament (ACL) reconstructed athletes. The intraclass relationship coefficients ranged between 0.93 and 0.98. Of the group, the standard errors of measurement and the smallest detectable differences were respectively, 2.6–4.1% and 7.2–11.3% of the mean hop distance. The proportion (%) of ACL-reconstructed individuals passing the ≥ 90% limb symmetry cut-off was 62.5 (single-hop for distance), 59.4 (triple hop for distance), 40.6 (medial side triple hop for distance) and 46.9 (medial rotation hop for distance). Hence, outstanding test-retest reliability of forward, medial and rotational hop tests was noted. This allows clinicians to make versed interpretations of variances in hop test distances when retesting athletes. Medial and rotational hop tests are more prone to exhibit limb asymmetries in ACL-reconstructed individuals in comparison with forward hop tests.