den Uil SH, van den Broek E, Coupé VMH, et al. - Researchers conducted this retrospective cohort study to assess the prognostic value of microvessel density (MVD), as a derived marker for angiogenesis, in stage II and III colon cancer and its relation to tumour-stroma-percentage and expression of hypoxia-inducible factor 1 (HIF1A) and vascular endothelial growth factor A (VEGFA). From 53 stage II and 54 (5-fluorouracil-treated) stage III patients, formalin-fixed paraffin-embedded colon cancer tissues were collected. By immunohistochemical evaluation of tissue microarrays, protein expression of HIF1A and VEGFA was determined. Investigators found that median MVD was higher in stage III vs stage II colon cancers. According to findings, MVD is an independent prognostic factor associated with poor disease free survival (DFS) in patients with stage II colon cancer and with improved DFS in patients treated with adjuvant chemotherapy with stage III colon cancer.