Zhang C, Dang D, Cong L, et al. - Researchers sought to determine the potential mechanisms underlying melanoma metastasis. They also explored prospective treatment targets for precise management of melanoma. They used the The Cancer Genome Atlas database to collect RNA-seq data (mRNA, miRNA, and lncRNA) for identifying hub genes in melanoma metastasis. Then, they validated the discovered hub genes in human tissues with qRT-PCR, followed by survival analysis. Using the CYBERSORTx, they ascertained the important immune cell type as well as its gene expression profiles in advanced melanoma microenvironment. Findings indicate a likely central role of elevated IL2RA, IL2RG, IL7R, and IFNG expression in melanoma metastasis promotion via upregulation of intratumoral regulatory T—cell proportion primarily by activation of JAK—STAT signaling pathway. Melanoma progression may be stimulated by LINC02446, LINC01857, and LINC02384 by decreasing tumor-protecting miR.891a.5p and miR.203b.3p. Also, a number of discovered molecules including TNFRSF(tumor necrosis factor receptor super family)13B, LAG3, NRP1, ENTPD1, NT5E, CCL21, and CCR7 can be future treatment targets in melanoma treatment.