Saá P, et al. - Researchers here focussed on the Food and Drug Administration released recommendations for individual-unit nucleic acid testing in 2016 to minimize the risk of transmission of Zika virus (ZIKV) through blood transfusions. Investigational screening of donated blood for ZIKV RNA by means of transcription-mediated amplification (TMA) was implemented by the American Red Cross. Confirmatory testing of reactive donations involved repeat TMA, TMA testing in exploratory minipools, real-time reverse-transcriptase polymerase chain reaction, IgM serologic testing, and red-cell TMA. Researchers noted screening of U.S. blood donations for ZIKV by individual-donation TMA as costly and had a low yield. They identified only 4 IgM-negative among the 9 confirmed ZIKV-positive donations; of these donations, all 3 that were tested were reactive on minipool TMA.