Bracks IV, et al. - In this study, researchers longitudinally analyzed the immune-inflammatory response in mice's teeth undergoing a regenerative protocol with or without the use of ethylenediaminetetraacetic acid (EDTA) to irrigate the root canal system. They divided teeth into the following groups: Empty – the canals were left empty; Blood Clot (BC) - the canals were filled with a blood clot; and EDTA + Blood - the canals were irrigated with 0.06 mL of 17% EDTA for 1 min and filled with a blood clot. According to results, the expression of IL-1 mRNA in the EDTA + BC group was significantly higher than in the Empty and BC groups during the 7th and 14th evaluation days. At all time periods, IL-10 mRNA expression was comparable across the 3 groups. Findings suggested that EDTA encouraged the increased expression of factors that could improve the outcome of regenerative endodontic therapy.