Wang L, Cheng HX, Zhou YH, et al. - Researchers inquired not only about the D-loop gene mutation and microsatellite instability in the mitochondrial DNA (mtDNA) but also regarding the correlation with the clinical and pathological parameters in laryngeal cancer. This analysis involved 60 cases of laryngeal cancer, from whom tumor tissues and paratumor tissues were obtained, and DNA was extracted from these tissues. In 51 (85%) of samples, there were 174 mutations across 38 sites. The majority of the mutations were concentrated in the high various (HV) I region, and the substitution of a single base or insertion and deletion of a single base were revealed as the main types of mutations. Presence of microsatellite instability in the D310 region was evident. Overall, findings revealed that high-frequency mutation of the D-loop gene in mtDNA was present in laryngeal cancer, which might have a crucial part in the pathogenesis of laryngeal malignancy.