Bibiana J Volta, Patricia L Bustos, Carolina González, et al. - Researchers here examined differentially expressed factors in Trypanosoma cruzi-infected pregnant women as potential markers of Chagas congenital transmission. ELISA or cytometric bead array was used to determine circulating levels of 12 cytokines and chemokines in T. cruzi-infected and uninfected pregnant women in their second trimester of pregnancy, and control groups of T. cruzi-infected and uninfected non-pregnant women. Overall findings suggest an increased probability of Chagas congenital transmission in correlation with high parasitemia together with low levels of TNF-α, IL-15, and IL-17, low TNF-α/IL-10 ratio, and high IL-12p70 levels. They suggest that T. cruzi-infected pregnant women who did not transmit the infection to their babies showed a different pro-inflammatory cytokine profile that might aid in predicting congenital transmission.