Baciu C, et al. - Given that during lung preservation and transplantation, ischemia/reperfusion injury (IRI) adds to primary graft dysfunction and poor clinical result, and it is essential to ascertain the biological mechanisms underlying IRI in order to inform the development of effective therapeutics, so, researchers explored metabolic profiling together with related alterations in gene expression to define biological pathways significantly modified at reperfusion vs cold ischemic time (CIT). Experts assessed metabolomics as well as transcriptomics profiles from 67 paired human lung tissue samples obtained from 2008-11 at the end of CIT and following 2 hrs of reperfusion. In integrative omics analysis, findings revealed increased expression of PNP (Purine Nucleoside Phosphorylase) gene as well as the generation of uric acid via the purine metabolic pathway. Experts identified a link of uric acid (urate) generation with oxidative stress—a prominent mechanism of IRI.