Tsou HH, et al. - Whether acrolein, a major cigarette-related carcinogen, is related to oral squamous cell carcinoma (OSCC) carcinogenesis, was investigated in this study including 97 OSCC cases and 230 healthy subjects with cigarette smoking (CS) and/or betel quid (BQ) chewing histories. Researchers carried out slot blot analysis of Acr-dG adducts (an indicator of acrolein-induced DNA damage in buccal DNA) as well as LC/MS-MS analysis of 3-HPMA levels, urinary Acr metabolites. Findings revealed 1.4-fold higher level of Acr-dG adducts in buccal cells in patients with OSCC vs in healthy individuals. Significantly higher levels of 3-HPMA were detected in healthy subjects in relation to CS and BQ chewing, this indicated the promotion of acrolein absorption by CS and BQ chewing. However, compared with healthy subjects, OSCC patients exhibited significantly lower 3-HPMA levels, suggesting impaired acrolein metabolism.