Pamuk B, et al. - A total of 52, euglycemic and euthyroid, obese females were evaluated to analyze the effect of weight loss with hypocaloric diet and orlistat treatment relevant to hypocaloric diet on gut-derived hormones ghrelin and obestatin. At the beginning and after 12 weeks of therapy anthropometric measurements, serum lipid, insulin levels, and obestatin and ghrelin values were estimated. Baseline clinical aspects and laboratory parameters including serum ghrelin, obestatin concentrations and ghrelin/obestatin ratio were assessed similar between the two groups categorized. After 12 weeks, mean change in BMI, fat mass, and fat-free mass (FFM) were observed in group 1. No correlation was observed between ghrelin or obestatin. They also analyzed that weight loss with diet and diet plus orlistat were both linked with increased ghrelin and obestatin concentrations.