Trico D, et al. - Researchers investigated whether in type 2 diabetes (T2D) patients the insulinotropic effect of a high-protein nutrient preload depends on the boost of plasma amino acids (AA) seen after digesting protein. Via the course of two 75-gram oral glucose tolerance tests preceded by either 500-ml water or a high-protein nutrient preload (50 grams of Parmesan cheese, one boiled egg, and 300-ml water), plasma AA levels were measured. They also assessed changes in beta cell function and changes in incretin hormone secretion. They reported an enhancement of beta cell function and glucagon-like peptide-1 (GLP-1) secretion in relation to elevated circulating AA that ensues following a high-protein nutrient preload. Overall, via a direct and GLP-1-mediated stimulation of insulin secretion by plasma AA, postprandial hyperglycemia may be attenuated by controlling the meal sequence of nutrient ingestion.