Bradshaw GL, et al. – Authors in this study describe the selection of two Borrelia burgdorferi–recombinant proteins and evaluation of their performance in a simple 1–tier test for the serological confirmation of Lyme Disease (LD). As per findings, these antigens could be used in a simple 1–tier ELISA assay that is faster to perform, easier to interpret, and less expensive than the 2–tiered test method, and which is better at detecting Borrelia specific antibodies in patient sera with stage I LD.