Wu Y, et al. - Researchers recruited 33 individuals with chronic prostatitis (CP) III and 30 healthy men in order to apply high‐throughput next‐generation sequencing to screen for potential pathogens correlated with NIH category III CP (CP III). This study conducted high‐throughput next‐generation sequencing to identify the sequence variations and the relative abundance of the bacterial 16S ribosomal variable region and fungal internal transcribed spacer region in all samples. They applied bioinformatics software and databases for data analysis, and differences with P < .05 were considered statistically significant. There was no potential pathogens in diagnosed patients with CP III. Before and after infection, the expressed prostatic secretions may be free from bacteria. Variations in the urinary tract microbiome may unsettle the microecological balance of the urinary system, thereby leading to CP III. It was considered that true pathogens of CP III may not be prokaryotic or eukaryotic microorganisms, further study is needed to evaluate the noncellular microbes.