Heidary Z, et al. - In this study, researchers tried to find out the expression of four recently reported mitochondrial-related miRNAs (miR-4485-3p/4484/4461 and 4463) in the sperm sample of asthenozoospermic men. RNA was obtained from spermatozoa of 74 volunteers (39 patients with idiopathic asthenozoospermia (AZS) and 35 controls with normal fertility), and by quantitative PCR, relative gene expression analysis was conducted. The SNORD48 was used as a normalizer gene, and quantification was estimated by the 2 ^−ΔΔCt method. The expression of miR-4484 and miR-4461 was not identified in the spermatozoa of cases and controls. The results showed that the downregulation of miR-4485-3p was correlated with idiopathic AZS, which could be a molecular connection between mitochondrial dysfunction and AZS.