Ortblad KF, Musoke DK, Chanda MM, et al. - Researchers sought to determine how knowledge of HIV-positive status affects the severity of depressive symptoms as it may assist in developing and delivering HIV testing interventions that will enhance the well-being, social functioning, and safety of FSW populations. Longitudinal data from two female sex worker (FSW) cohorts in Uganda (n = 960) and Zambia (n = 965) who had ample opportunity to HIV testing using standard-of-care services or self-tests over 4 months were obtained. Participants' perceived knowledge of HIV status, severity of depressive symptoms (continuous PHQ-9 scale, 0–27 points), and prevalence of likely depression (PHQ-9 scores ≥ 10) were determined at baseline and 4 months. The analysis revealed a significant association of knowledge of HIV status, be it positive or negative, with a reduction in depressive symptoms in 2 FSW populations. FSWs may attain mental health benefits with the expansion of HIV testing programs.