Scientists at the UNC School of Medicine have identified and isolated a protein that could play a vital role in regulating proper airway function. When that protein is low in volume or missing altogether, it can cause airway hyper–reactivity in asthma.
Researchers at the UNC School of Medicine believe they have isolated a protein that, when missing or depleted, can cause airway constriction, production of mucus, chest tightness, and difficulty breathing for the 334 million people worldwide who suffer from asthma.

And they’re hopeful that this discovery, published in the journal Nature Communications, will lead to more effective treatments for asthma.

Robert Tarran, PhD, associate professor of medicine, and a member of the UNC Marsico Lung Institute, primarily focuses his research on cystic fibrosis and chronic obstructive pulmonary disease. But after identifying a protein – SPLUNC1 – in cystic fibrosis research, Tarran’s colleague Steve Tilley, MD, UNC associate professor of medicine, wondered what role it might play in asthmatics.

“We first measured SPLUNC1 levels in airway samples obtained from asthmatics and normal volunteers in the UNC Center for Environmental Medicine, Asthma, and Lung Biology,” Tilley said. “We were astonished to find that SPLUNC1 levels were markedly reduced in people who have asthma.”

Using mouse models that were given allergens similar to people who suffer from asthma, Tilley’s lab found that SPLUNC1 levels were depleted in the airways, similar to the findings in humans with asthma, and that restoring SPLUNC1 reversed airway hyper–responsiveness, which is a cardinal feature of asthma. Tarran’s lab determined that SPLUNC1 could regulate contraction of the airway smooth muscle by preventing a calcium entry into smooth muscle cells, providing a mechanistic explanation of how a deficiency of this protein might lead to airway hyper–responsiveness.

Epithelial cells that line airways produce the SPLUNC1 protein.

“We found that this protein, which is actually turned off by excessive inflammation, is needed to cause the muscle to relax. It’s essentially a muscle–relaxing factor that’s missing from asthma patients. It’s something that normally acts as a brake,” Tarran said.

A potential therapy for asthma would be to replenish either the whole protein or part of the protein, which could be delivered via a nebulizer or inhaler. Matt Redinbo, PhD, a professor of biochemistry and biophysics, and microbiology and immunology at the UNC School of Medicine, identified the crystal structure of SPLUNC1, which Tarran said was key in developing the next steps of this research.

“Since we know the crystal structure of the protein, we’re able to find the active site of the protein that regulates smooth muscle contraction,” Tarran said. “So we can make peptides or drugs to target that active site and see if that works. That’s one approach.”

Tarran and Tilley have submitted a National Institutes of Health grant to study this hypothesis in patients.

“We want to study this in patients to correlate SPLUNC1 levels with airway hyper–reactivity,” Tarran said. “And we also want to go deeper into the mechanism – how does this protein do what we observed. So there are several future avenues of research: expanding clinical studies, designing drugs in mouse studies, and then studying the underlying biology of what happens in a person with asthma.” “If we can further establish that SPLUNC1 is the elusive epithelial–derived relaxing factor that is deficient in asthmatics, then we can begin working on ways to restore SPLUNC1 levels in patients as a novel therapy to treat asthma,” Tilley said. “I am looking forward to working with Drs. Neil Alexis, Ilona Jaspers, and David Peden in our asthma center to design more translational studies in humans so that we can determine the clinical significance and therapeutic