House dust mites may be carriers for IgE sensitization in dermatitis
Medical University of Vienna News Aug 30, 2017
For patients with atopic dermatitis (AD), house dust mites (HDMs) may act as carriers for immunoglobulin E (IgE) sensitization to microbial antigens, according to a study published online July 25 in the journal Allergy.
Sheron Dzoro, from the Medical University of Vienna, and colleagues used IgE immunoblotting to analyze plasma samples from 179 AD patients for IgE–reactivity to a panel of micro–arrayed HDM allergen molecules and to Staphylococcus aureus and Escherichia coli. The authors tested antibodies specific for S. aureus and E.coli antigens for reactivity to nitrocellulose–blotted extract from purified HDM bodies, and detected IgE–reactive antigens by IgE–immunoblot inhibition experiments. IgE ImmunoCAP inhibition experiments were performed to quantify IgE antibodies directed to bacterial antigens in HDMs.
The researchers found that compared to AD patients without HDM sensitization, IgE–reactivity to bacterial antigens was significantly more frequent in AD patients sensitized to HDMs. In immune–blotted HDM extract, S. aureus and E. coli antigens were detected, while qualitative and quantitative IgE inhibition experiments demonstrated the presence of IgE–reactive antigens in HDMs.
ÂHDMs may serve as carriers of bacteria responsible for the induction of IgE sensitization to microbial antigens, the authors wrote.
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Sheron Dzoro, from the Medical University of Vienna, and colleagues used IgE immunoblotting to analyze plasma samples from 179 AD patients for IgE–reactivity to a panel of micro–arrayed HDM allergen molecules and to Staphylococcus aureus and Escherichia coli. The authors tested antibodies specific for S. aureus and E.coli antigens for reactivity to nitrocellulose–blotted extract from purified HDM bodies, and detected IgE–reactive antigens by IgE–immunoblot inhibition experiments. IgE ImmunoCAP inhibition experiments were performed to quantify IgE antibodies directed to bacterial antigens in HDMs.
The researchers found that compared to AD patients without HDM sensitization, IgE–reactivity to bacterial antigens was significantly more frequent in AD patients sensitized to HDMs. In immune–blotted HDM extract, S. aureus and E. coli antigens were detected, while qualitative and quantitative IgE inhibition experiments demonstrated the presence of IgE–reactive antigens in HDMs.
ÂHDMs may serve as carriers of bacteria responsible for the induction of IgE sensitization to microbial antigens, the authors wrote.
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